Other
subE
Part:BBa_K2020000:Design
Designed by: Nicola Freyer, Lea Steinbeck, Svenja Meyer, Alexander Deitert Group: iGEM16_Aachen (2016-10-04)
subtilisin E gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence from B. subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT.
Source
The sequence wasoriginally obtained from a wildtype Bacillus subtilis and was ordered from IDT.