Part:BBa_K2009357:Design
SUP35
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 522
Design Notes
The SUP35NM gene we used is provided by Dong Men, PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences, so that the sequence is not quite the same as the existing sequence in the Parts Registry, for a lot of mutations have been done. The standardization of this gene is did by ourselves by adding the Biobrick prefix and suffix to its ends and doing a site mutation to eliminate the PstI cutting site inside it.
Source
PCR from the plasmid provided from Dong Men , PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences
References
The SUP35NM gene we used is originally provided by Dong Men, PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences, and the sequence is not quite the same as the existing sequence in the Parts Registry, for a lot of mutations have been done. The standardization of this gene is did by ourselves by adding the Biobrick prefix and suffix to its ends and doing a site mutation to eliminate the PstI cutting site inside it. Comparing to the sequence submitted by other team, our SUP35 gene is shorter, which means lower expressing pressure and the possibility to express more protein in the E.coli.