Generator

Part:BBa_K1961004:Design

Designed by: Yi-Ting Lin   Group: iGEM16_HSiTAIWAN   (2016-10-03)
Revision as of 23:35, 3 October 2016 by Jonathan0912 (Talk | contribs) (→‎Source)


PbrR generator expressed by GFP under autoregulation of PTet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2040


Design Notes

This sequence consists of two sub-parts: (1) TetR generator under under control of PTet, which has TetR binding sites; (2) PbrR generator expressed by GFP under control of PTet. We used tetracycline to induce the transcription.

Source

We use parts in the 2016 iGEM plate R0040, B0034, C0040, B0010, B0012, R0040, B0034, I721002, E0040, B0010, B0012

References