Protein_Domain

Part:BBa_K2017001

Designed by: Monica Victoria Gutierrez Salazar   Group: iGEM16_Valencia_UPV   (2016-10-03)
Revision as of 13:36, 3 October 2016 by Registry (Talk | contribs)

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N-split Cas9 + DnaE N-intein

Half of the SpCas9 protein fused to DnaE C-intein. The active full-lenght spCas9 can be recovered by using the other half of the protein, C-split Cas9 + DnaE C-intein. Inteins are used to fuse both parts of the protein, as they interact and splice, leaving the active protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 268
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 268
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
    Illegal BglII site found at 1212
    Illegal BglII site found at 2192
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 268
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/nbenthamiana
//function/crispr
Parameters
None