Part:BBa_K2036024

placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase

Whole circuit of the application of our prokaryotic signal filter.
"Main function":
We construct this circuit to apply our prokaryotic signal filter.
As the figure shows, Clll(BBa_K2036001) can combine with Ftsh and thus competitively preventing the degradation of Cll and Cll can activate the promoter pRE.
When body intake large amount of lactose .The lactose signal as pulse signal induce the transcription of downstream components. Then cro, Cll and Clll will have a low and transient expression level , these parts form a positive feedback system and direct stable expression of Cro, Cro binds to cro binding site and blocks the pRM promoter so that only gene iLDH will be expressed and can transform lactic acid into pyruvate.
When PH is in the range of 7~9 , Cl will be expressed and bind to Cl binding site ,then block the expression of iLDH nd Clll, which will shut down positive feedback and lead to the expression of β-galactosidase and the degradation of lactose.

Whole circuit of the application of prokaryotic signal filter.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 3212
  Illegal BamHI site found at 3251
  Illegal BamHI site found at 3943
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]