Composite

Part:BBa_K2036024:Design

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-20)
Revision as of 09:48, 3 October 2016 by Shareycheng (Talk | contribs) (→‎Source)

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placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3212
    Illegal BamHI site found at 3251
    Illegal BamHI site found at 3943
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a lactic acid balance circuit in E.coli with lactose signal to initiate. And when the pH environment are within 7 to 9,the host strain will prepare beta-galactosidase for next lactose input.
beta-galactosidase coding gene are alpha domain of laz,it functions only in E.coli strain which contains lacZ operon.

Source

CI,CII,CIII,Cro,pRE,pR,pRM are derived from bacteriaphage lambda operon. And placm, iLDH, patp2 are from the Registry. Beta-galactosidase coding sequence are reffered to pUC119 commerial vector

References