Composite

Part:BBa_K2036023

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-20)
Revision as of 08:43, 3 October 2016 by Shareycheng (Talk | contribs)


RBS-iLDH-TT-pRM-RBS-beta-galactosidase

This part is built by In-Fusion cloning method and together with BBa_K2036021 and BBa_K2036023 to further assemble BBa_K2036024. pRM can be negatively regulated by Cro. ilDH and beta-glactosidase are important enzyme in celluler metobolism.

Fig1: Prokaryote application plasmid for lactose intolerance

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 737
    Illegal BamHI site found at 776
    Illegal BamHI site found at 1468
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None