Coding
tsPurple

Part:BBa_K1033906

Designed by: Sabri Jamal   Group: iGEM13_Uppsala   (2013-08-25)
Revision as of 08:43, 3 October 2016 by Saishreyas g (Talk | contribs)

tsPurple, purple chromoprotein

This chromoprotein (also known as TinselPurple) naturally exhibits strong purple color when expressed.

Usage and Biology

This part is useful as a reporter.

AmilCP and tsPurple.jpg

iGEM2013 Uppsala: The images above show E coli constitutively expressing the chromoproteins amilCP BBa_K592009 and tsPurple BBa_K1033906 from the high copy plasmid pSB1C3 from the promoters J23116 and J23110.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Structure and SWISS MODEL Homology Modelling Report

(The following information is the contribution of SVCE_Chennai)

The following sections give a detailed information on this chromoprotein done by its in silico analysis.

3D Structure

Ts2.gif

Cartoon representation of tsPurple.

Ts purple swiss.png


QMEANnorm = 0.02
Cβ = -0.91
All Atom = 0.52
Solvation = -1.19
Torsion = 0.32


The best match during structure prediction.

Template Seq. Identity Oligo-state Found by Method Resolution Seq Similarity Range Coverage Description
4ohs.1.A 86.61 homotetramer HHblits X-Ray 2.19Å 0.58 2 - 222 0.98 FAR-RED FLUORESCENT PROTEIN AQ143


What about Ligands?

Ligand Added to Model Description
CL ✕ - Not biologically relevant. CHLORIDE ION
CL ✕ - Not biologically relevant. CHLORIDE ION
CL ✕ - Not biologically relevant. CHLORIDE ION



Tsp2.png



Tsp.png



Tsp3.png



Tsp4.png

[edit]
Categories
//cds/reporter/chromoprotein
//collections/chromoprotein/uppsala
//function/reporter/pigment
Parameters
colorPurple