Composite

Part:BBa_K2036022

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-20)
Revision as of 08:30, 3 October 2016 by Shareycheng (Talk | contribs)


patp2-RBS-CI-TT-pR-RBS-CIII

This part is pH sensitive on account of pH sensitive promoter patp2. CI and pR are derived from bacteria lmabda operon. CI can block polymerase within pR so that transcription of CIII is inhibited. So it forms a NO gate of CIII expression. It serves as an intermediate to construct Prokaryote application circuit of Signal Filter([http://2016.igem.org/Team:HUST-China see HUST-China 2016 project]). This part is built by In-Fusion cloning method and together with BBa_K2036021 and BBa_K2036023 to further assemble BBa_K2036024

Fig1: Prokaryote application plasmid for lactose intolerance

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None