Composite

Part:BBa_K2092009

Designed by: Hui Wen Chong   Group: iGEM16_Manchester   (2016-10-02)
Revision as of 23:28, 2 October 2016 by Huiwenchong (Talk | contribs)

PalcA(var) + RBS (B0034) +amilCP The alcA promoter, PalcA, is originally found in Aspergillus nidulans as a part of the ethanol regulon. PalcA requires its positive transcriptional regulator AlcR to regulate the expression of gene alcA. Gene alcA encodes for alcohol dehydrogenase I (ADHI) which facilitates the interconversion between alcohols and carbonyls. This part consists of a PalcA (BBa_K2092002, https://parts.igem.org/Part:BBa_K2092002) variant tagged with a blue chromoprotein, which makes it capable to produce blue colour upon the binding of its ethanol-activated transcription factor AlcR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None