RNA

Part:BBa_K2020042

Designed by: Andrea Hoeltken, Vroni Czotscher, Carolina Bonerath   Group: iGEM16_Aachen   (2016-10-02)
Revision as of 18:34, 2 October 2016 by Andrea hoeltken (Talk | contribs)


Mj-tRNA with amber anticodon for incorporating ncAA in E.coli For incorporating unnatural amino acids into a protein, a orthogonal tRNA:Synthetase-pair is needed which does not crossreact with the cognate tRNA:Synthetase-pairs. This tRNA can be assembled with a variety of synthetases into a plasmid to incorporate ncAA in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None