RNA
Part:BBa_K2020042:Experience
Designed by: Andrea Hoeltken, Vroni Czotscher, Carolina Bonerath Group: iGEM16_Aachen (2016-10-02)
Revision as of 13:51, 2 October 2016 by Andrea hoeltken (Talk | contribs)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2020042
User Reviews
UNIQ7c5c0f12dbb30a70-partinfo-00000000-QINU UNIQ7c5c0f12dbb30a70-partinfo-00000001-QINU
Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a low copy plasmid (e.g. pACYC) which fits your needs.
[http://2016.igem.org/Team:Aachen Team Aachen 2016] used tRNA in pACYC with p15A-ORI, Ipp-Promoter, Terminator, Gent-Resistance. Together with an ncAA-Synthetase (For example: BBa_K2020050) with own promoter and Terminator.