RNA

Part:BBa_K2020042

Designed by: Andrea Hoeltken, Vroni Czotscher, Carolina Bonerath   Group: iGEM16_Aachen   (2016-10-02)
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Mj-tRNA with amber anticodon for incorporating ncAA in E.coli

For incorporating unnatural amino acids into a protein, a orthogonal tRNA:Synthetase-pair is needed. This tRNA derives from wild type tyrosyl Methanococcus janaschii tRNA:Synthetase pair. It is used together with a tRNA-Synthetase in E.coli. The tRNA has an amber anticodon for incorporating the ncAA in response to an amber codon. It has been proven to work with (enter links for parts) wild type Y-RS, AzF-RS, CNF-RS, Iodo-Y-RS, 5HTP-RS, Nitro-Y-RS by iGEM-Team Texas 2014 (Link).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None