Regulatory

Part:BBa_K2092002:Design

Designed by: Hui Wen Chong   Group: iGEM16_Manchester   (2016-10-01)
Revision as of 16:03, 1 October 2016 by Huiwenchong (Talk | contribs) (→‎Design Notes)


PalcA, improved alcR inducible promoter from A. nidulans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

XbaI and SpeI restriction sites were missing in the original BioBrick by 2011 iGEM DTU-Denmark. To add the following sites, two primers were designed:

alcA forward primer = 5'- CGG AAT TCG CGT CTA GAA CGT CGC TCT CCC CGA TGA C -3' alcA reverse primer = 5'- AAC TGC AGA AAC CAA TGC ATT GGA CTA GTT TTT GAG GCG AGG TGA TAG GAT TGG -3'

Source

Aspergillus nidulans, sequence found through

References