Coding

Part:BBa_K401001:Experience

Designed by: Daniel Tamarit   Group: iGEM10_Valencia   (2010-10-21)
Revision as of 03:15, 1 October 2016 by Verweile (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K401001

User Reviews

UNIQ0c25c5b166f903ef-partinfo-00000000-QINU UNIQ0c25c5b166f903ef-partinfo-00000001-QINU

Improvement by USTC 2016


Our part is Part: BBa_K2009357 .The SUP35NM gene we used is originally provided by Dong Men, PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences, and the sequence is not quite the same as the existing sequence in the Parts Registry, for a lot of mutations have been done. The standardization of this gene is did by ourselves by adding the Biobrick prefix and suffix to its ends and doing a site mutation to eliminate the PstI cutting site inside it. Comparing to the sequence submitted by other team, our SUP35 gene is shorter, which means lower expressing pressure and the possibility to express more protein in the E.coli.