Regulatory
GAL1
Part:BBa_J63006
Designed by: Caroline Ajo-Franklin Group: Silver Lab (2006-10-11)
yeast GAL1 promoter
GAL1 promoter from S. cerevisiae Contains Kozak sequence, part BBa_J63003.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]
Promoter characterization
Team Czech Republic 2015 used this promoter very extensively. As a result, the characterization of this part was improved. See the Experience page for details.
Promoter Strength
JHU Igem 2011 is working on further characterization of this part through using GFP expression within the next month.
Promoter improvement
Team USTC 2016 modify this part to make it "ready to use". It is more convenient to be utilized now. See the Experience page for details.
Promoter dose =
Gal1 prevents growth in media containing galactose.
SCUT iGEM 2014 have designed a experiment to quantify Gal1 dose titer.
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Categories
Parameters
//rnap/eukaryote/yeast
//direction/forward
//chassis/eukaryote/yeast
//promoter
//regulation/positive
//direction/forward
//chassis/eukaryote/yeast
//promoter
//regulation/positive
negative_regulators | |
positive_regulators | 1 |