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Part:BBa_I716001:Design

Designed by: Arthur Yu   Group: iGEM07_Berkeley_UC   (2007-06-06)
Revision as of 19:06, 6 June 2007 by Ayu (Talk | contribs) (→‎Design Notes)

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neuS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 100


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

Bbrick ORF neuS
[pcr] neus-F/R on pJ23006-Bca9106        (1261bp, BglII/XhoI)
[sub] into pBca9145-Bca1144#5            (BglII/XhoI, 2063+910, L)
[prod] pBca9145-neuS (I716001)

---
AY003.neus-F     ctacgAGATCTatgatatttgatgctagtttaaag
AY004.neus-R     gtcagCTCGAGttaGGATCCttactcccccaagaaaatcc

Source

pJ23006-Bca9106

References