Coding

Part:BBa_K2003012:Design

Designed by: Tora Hävermark   Group: iGEM16_Uppsala   (2016-09-29)
Revision as of 16:59, 29 September 2016 by Registry (Talk | contribs)

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Inducible Green Fluorescent Protein UnaG+Flexible Linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 437
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original UnaG sequence was ordered from IDT with a 6xhistidine tag on the 5' end and a flexible linker on the 3' end. However, the first two codons before the flexible linker were stop codons, meaning that the default UnaG does not translate the flexible linker. The histidine tag and the stop codons were removed from the sequence by PCR, resulting in a UnaG sequence without the histidine tag and with the flexible linker.


Source

Synthesized by PCR mutagenesis from UnaG sequence designed and ordered from IDT.

References