Part:BBa_K2052014

FimH site directed mutated with RPMrel

Function: Some substrains of E.Coli have structure called Type 1 pili which is expressed from the Fim gene system. At the end of the pili structure there is a protein called “FimH” which is the structe that allows them to bind to the mannose sugar that is found on the surfaces of eukaryotic cells. (Sauer et al., 2016).However, the substrain that was used in this project was BL21, a non-pathogenic substrain. So, it does not bind to eukaryotic cells. Rpmrel, is a tumor specific binding peptide (Kelly et al., 2003). When combined with FimH it can bind to the tumor cells in the colon flora. ....Enter a long description of the part so that users of your part know what it is, what it does, and how to use it in their projects.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1173
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1113
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]