Composite

Part:BBa_K2036019

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-20)
Revision as of 07:42, 29 September 2016 by Shareycheng (Talk | contribs)


RFP-LAAtag-TT-pRM-RBS-GFP-LVAssrAtag

This is a part of Prokaryote version of Signal Filter ([http://2016.igem.org/Team:HUST-China Details see to HUST-China 2016 wiki]) characterization circuit:pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag (BBa_K2036027).

Fig1:Prokaryote version of Signal Filter characterization circuit


Because the circuit is too complex, we divided it into three parts and each is assembled by In-Fusion method (homologous recombination). The whole circuit is built by 3A assembly and then we transfer the backbone from pSB1C3 to PET-Duet-1.

Fig2:Prokaryote version of Signal Filter characterization plasmid

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1601


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Categories
Parameters
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