Project
Part:BBa_K2036015
Designed by: Zhangyu Cheng Group: iGEM16_HUST-China (2016-09-19)
Revision as of 05:15, 29 September 2016 by Shareycheng (Talk | contribs)
RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag
CII acts as a necessary and sufficient cis-acting target for rapid proteolysis by initiating pRE. The circuit is built to test CII and pRE interaction together with RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag( BBa_K2036013)and pRE-RBS-GFP-LVAssrAtag( BBa_K2036011) . Considering Ftsh protease degrades it raidly in the host, RBS-CII-RBS-CII tandom expression circuit is constructed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1770
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Categories
Parameters
None |