Project

Part:BBa_K2036013

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-19)
Revision as of 08:05, 28 September 2016 by Shareycheng (Talk | contribs)


RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag

It is a GFP regulationary circuits. When CII is expressed it can further activate GFP and forming a Switching Lag-times. HUST-China 2016 build this part to characterize CII and pRE interaction with a control group:pRE-GFP-LVAssrAtag ( BBa_K2036011). And we also construct a tandem expression of CII (BBa_K2036015)to figure out if the flouresence level will come up with CII.

Fig1:CII&pRE interaction characterization circuits

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1168


[edit]
Categories
Parameters
None