Composite

Part:BBa_K2036011

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-19)
Revision as of 07:52, 28 September 2016 by Shareycheng (Talk | contribs)


pRE-GFP-LVAssrAtag It is a GFP generator,and the production of GFP will be activated by a certain level of CII or CII with help of CIII in E.coli. HUST-China 2016 built this circuit to characterize CII and pRE interaction with test group:RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036013)and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036015)

Fig1:CII&pRE interaction characterization circuit

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 719


[edit]
Categories
Parameters
None