Composite
Part:BBa_K1895999
Designed by: Jake Burton Group: iGEM16_Newcastle (2016-07-21)
Revision as of 18:28, 27 September 2016 by Jakeb (Talk | contribs) (Add description and basic biology of Arabinose controlled resistor.)
Arabinose controlled 'resistor'
As part of our iGEM project we have attempted to engineer E. coli to behave like a variable resistor. We hoped to do this by using E. coli to vary the amount of free ions in an electrolyte. Ion uptake will be controlled by the expression of a protein, smtA. SmtA is a metallothionein that can bind to heavy metal ions like cadmium (II), Zinc (II) and Copper (II).
In this construct we have placed smtA under the control of an AraC regulated promoter allowing the expression of smtA to be controlled by the addition or removal of arabinose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 103
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 338
Illegal AgeI site found at 674 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
None |