DNA

Part:BBa_K2100002:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-09-26)
Revision as of 23:13, 26 September 2016 by Registry (Talk | contribs)

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pENTR pEREx6


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
    Illegal XbaI site found at 42
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
    Illegal XbaI site found at 42
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
    Illegal XbaI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Like TRE yay


Source

Synthesized by IDT. Sequence given b/n L4 R1 sites

References