Coding

Part:BBa_K2036005

Designed by: Wangjie Liu   Group: iGEM16_HUST-China   (2016-09-19)
Revision as of 08:49, 25 September 2016 by WangjieLiu (Talk | contribs)


PP2CA,a type of protein from subfamily of type 2C protein phosphatases

Abscisic acid (ABA) is an essential hormone for plants to survive environmental stresses. As one of important part in ABA signaling network , PP2CA can form exclusive interactions with ABA receptors and subfamily 2 Snfl-related kinase. Also it can directly dephosphorylate the ABFs transcription facrors. These two functions both lead to inactivation of the Snf1-related(SnRK)2 clade of protein kinases, and inactivation of their downstream targets such as ABA-response element binding basic leucine zipper (bZIP) transcription factors. Application in project: we apply PP2CA into our eukaryotic filter .When pulse OFF appears,PP2CA can dephosphorylate ABF2 and inhibite the function of SnRK2.2,these both block the expression of genes downstream the RD29A promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 90
    Illegal BamHI site found at 661
    Illegal BamHI site found at 806
    Illegal BamHI site found at 918
    Illegal XhoI site found at 65
    Illegal XhoI site found at 875
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None