Device

Part:BBa_K1920006

Designed by: Chia-En,Wong   Group: iGEM16_NCKU_Tainan   (2016-09-23)
Revision as of 01:42, 24 September 2016 by Registry (Talk | contribs)

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pBAD-RBS-lysis-B0015

pBAD is an E.coli promoter that is induced by L-arabinose.In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription [1]. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription [1].[***] However, under the higher concentration of glucose environment, pBAD will be inhibited[***]. But there is still a proper range of glucose concentration, allowing us to control the expression of the lysis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None