Coding

Part:BBa_K1927000:Design

Designed by: Marthe Jrgensen   Group: iGEM16_UiOslo_Norway   (2016-09-23)
Revision as of 11:43, 23 September 2016 by Registry (Talk | contribs)

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Beta-lacatamase enzyme called blaNDM-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 716


Design Notes

We added prefix and suffix to the gene so that we could perform restriction digestion and Gibson Assembly. We had to design the gene so that it will get the correct readingframe.


Source

The sequence is collected from pUC19 vector online and sent for synthesis to IDT.

References