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Coding
Part:BBa_K2042002
Designed by: Maria Bargués i Ribera Group: iGEM16_Evry (2016-09-02)
Revision as of 17:49, 20 September 2016 by RATOVOMANANA (Talk | contribs)
Pseudomonas sp. MBEL 6-19 PHA synthase 1
This is the coding sequence for a Polyhydroxyalkanoate synthase from Pseudomonas sp. MBEL 6-19. The enzyme takes the monomer (D)-lactyl-CoA and produce the PLA homopolymer. This sequence is codon optimized for Pseudomonas putida and includes four amino acid mutations (E130D/S325T/S477G/Q481K) which were found to increase yields of PLA. This type II PHA synthase 1 accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA). We tested different PhaC from different organism to compare the difference of expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1060
Illegal NgoMIV site found at 1339
Illegal AgeI site found at 135
Illegal AgeI site found at 448 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//cds/biosynthesis
//chassis/prokaryote/pputida
//chassis/prokaryote/pputida
None |