Coding

Part:BBa_K2042002

Designed by: Maria Bargués i Ribera   Group: iGEM16_Evry   (2016-09-02)
Revision as of 17:49, 20 September 2016 by RATOVOMANANA (Talk | contribs)


Pseudomonas sp. MBEL 6-19 PHA synthase 1

This is the coding sequence for a Polyhydroxyalkanoate synthase from Pseudomonas sp. MBEL 6-19. The enzyme takes the monomer (D)-lactyl-CoA and produce the PLA homopolymer. This sequence is codon optimized for Pseudomonas putida and includes four amino acid mutations (E130D/S325T/S477G/Q481K) which were found to increase yields of PLA. This type II PHA synthase 1 accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA). We tested different PhaC from different organism to compare the difference of expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
    Illegal NgoMIV site found at 1339
    Illegal AgeI site found at 135
    Illegal AgeI site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//chassis/prokaryote/pputida
Parameters
None