Composite

Part:BBa_K2036025:Design

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-20)
Revision as of 05:53, 20 September 2016 by Registry (Talk | contribs)

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Kozak-Snrk2.2-TTADH1-pCyc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 15
    Illegal BamHI site found at 672
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a lactic acid balance circuit in E.coli with lactose signal to initiate. And when the pH environment are within 7 to 9,the host strain will prepare beta-galactosidase for next lactose input.


Source

derived from bacteriaphage lambda operon. And placm, iLDH, patp2 are from the Registry. Beta-galactosidase coding sequence are reffered to pUC119 commerial vector

References