Part:BBa_K2033000:Design

N-dodecanoyl-L-homoserine lactone (C(12)-HSL) Sender- AubI

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 535
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 510
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The original AubI part was sampled from soil and is thought to be from an unknown soil-borne bacteria. The bacteria was transformed into BL21 Competent E. coli cells to produce sufficient stock for future experiments. Of the 70 colonies that were produced, three samples were taken for verification. After mini-prepping, the gel verification affirmed the identity of the sample, as shown below:

Lanes 3,4,5 correspond to AubI samples; Lane 1 contains the kb+ ladder.

Source

This synthase was recovered from a metagenomic project on a soil sample. The specific source is unknown.

References

(1) Nasuno, E., N. Kimura, M. J. Fujita, C. H. Nakatsu, Y. Kamagata, and S. Hanada. "Phylogenetically Novel LuxI/LuxR-Type Quorum Sensing Systems Isolated Using a Metagenomic Approach." Applied and Environmental Microbiology 78.22 (2012): 8067-074. Web