Coding

Part:BBa_K2036005:Design

Designed by: Wangjie Liu   Group: iGEM16_HUST-China   (2016-09-19)
Revision as of 06:12, 19 September 2016 by Registry (Talk | contribs)

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PP2CA,a type of protein from subfamily of type 2C protein phosphatases


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 90
    Illegal BamHI site found at 661
    Illegal BamHI site found at 806
    Illegal BamHI site found at 918
    Illegal XhoI site found at 65
    Illegal XhoI site found at 875
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is successfully expressed in yeast to test its interaction with ABRE DNA sequence


Source

come from Arabidopsis Thaliana, function as a transcriptional activator

References