Coding

Part:BBa_K1961000:Design

Designed by: Yi-Ting Lin   Group: iGEM16_HSiTAIWAN   (2016-09-18)
Revision as of 13:50, 18 September 2016 by Jonathan0912 (Talk | contribs) (→‎References)

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CYP2A13 is a member of the Cytochrome P450 (CYP) enzymes family, which are critical for the metaboli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 777
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 910
    Illegal BsaI.rc site found at 1199
    Illegal SapI site found at 327
    Illegal SapI.rc site found at 81


Design Notes

In the whole sequence, our team replaced the sequence CTGCAG(PstI)with CTCCAA.



Source

Our team obtained this sequence from the NCBI(NCBI Reference Sequence: NM_000766.4), Homo sapiens cytochrome P450 family 2 subfamily A member 13.

http://www.ncbi.nlm.nih.gov/nucleotide/371873114report=genbank&log$=nuclalign&blast_rank=1&RID=XX4XFCXN016&from=22&to=1506

References

Xiao-Yang He, Lili Tang, Shou-Lin Wang, Qing-Song Cai, Jia-Sheng Wang and Jun-Yan Hong. Efficient activation of aflatoxin B1 by cytochrome P450 2A13, an enzyme predominantly expressed in human respiratory tract. Int. J. Cancer 2006 (118):2665–2671