Inverter

Part:BBa_K2012011

Designed by: Pan Chu   Group: iGEM16_HZAU-China   (2016-09-15)
Revision as of 06:16, 15 September 2016 by Registry (Talk | contribs)

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J23117-Bc345

Tandem rioswitches bc345 are located in the promoter J23117 upstream. It can control gene expression. There are low leakage expression of gene which you interest in E.coli whose concentration of c-di-GMP is in a normal level. When cell is induced expression gene which can catalyze c-di-GMP from GTP, the tandem riboswitches would trans to open form and express its downstream gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rna/riboswitch
Parameters
None