Reporter

Part:BBa_K2009363:Design

Designed by: Chengle Zhang   Group: iGEM16_USTC   (2016-09-14)
Revision as of 14:26, 14 September 2016 by Registry (Talk | contribs)

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PGAL+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1119


Design Notes

The designer of Part:BBa_J63006 is very thoughtful for adding the KOZAK sequence to the downstream of the GAL1 promoter. Apparently, they meaned to improve the effeiciency of this part. However, considering the existence of the Biobrick prefix, the ATG inside the KOZAK sequence won't be in the same reading frame as the ATG of the downstream coding sequence. There were two teams managed to fix this problem, with the idea to eliminate the ATG inside the KOZAK sequence. This year, we applyed a different idea. We added one base pair between those two ATGs and made them belong to one common frame. Meanwhile, another ATG is made up between the former ones, belonging to the same frame.


Source

We construted this composite parts by ligating two former existing parts and doing a site mutation. The two parts we used is (1) GAL1 promoter Kozak sequence (Part:BBa_J63006) and (2) GFP (Part:BBa_E0040).

References