Composite

Part:BBa_K1905003

Designed by: Juan Carlos Rueda Silva   Group: iGEM16_TecCEM_HS   (2016-09-13)
Revision as of 03:15, 14 September 2016 by Registry (Talk | contribs)

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Riboswitch to detect E5 mRNA from HPV-16 and express RFP

DNA coding for a riboswitch to detect E5 mRNA from Human Papillomavirus type 16 and express Red Fluorescent Protein. This part includes a promoter (BB_R0010) and a RBS (BBa_B0034), the reverse complementary for a section of E5 DNA coding region, and the original sequence with one base changed, RFP coding region (BBa_E1010) and a terminator (BBa_B0015). When there is presence of E5 mRNA, the riboswitch turns on and it allows the expression of RFP as reporter protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 838
    Illegal AgeI site found at 950
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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