![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K2100000
Designed by: Kathleen Brandes Group: iGEM16_MIT (2016-09-12)
pENTR pEREx3
pEREx3 is a synthetic mammalian promoter that responds to estrogen receptor activated by estradiol (E2). pEREx3 consists of three repeats of the estrogen response element (ERE) consensus sequence upstream of a minimal promoter (minCMV).
We have demonstrated a 10-fold activation in MCF7 cells treated with nanomolar E2/EtOH concentrations when cascaded with eYFP.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//awards/part_collection/2016
None |