Coding
Part:BBa_K1954000
Designed by: Kamil Zmijewski Group: iGEM16_UCL (2016-09-12)
LanA
This entry is the open reading frame for the mutacin 1140 prepropeptide from Streptococcus mutans JH1140. Mutacin 1140 is type A lantibiotic (a linear antimicrobial peptide) differing from other bacteriocins by the presence of distinct post-translational modifications. The mature mutacin peptide is a screw-shaped molecule that interferes with the longevity of Gram-positive bacteria by means of membrane perturbation.
In order for mutacin 1140 to be functional the action of modifying enzymes is required. These enzymes catalyze the formation of stereospecific thioether bridges, dehydration, decarboxylation of the core peptide and leader peptide cleavage, as well as act as exporters of the modified mutacin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 57
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |