Composite

Part:BBa_K1890002

Designed by: Lycka Kamoen, Maria Vazquez   Group: iGEM16_TU_Delft   (2016-09-08)
Revision as of 12:25, 8 September 2016 by Registry (Talk | contribs)

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Silicatein gene, fused to transmembrane domain of OmpA, with strong RBS

Silicatein, originating from the demosponge Tethya aurantia, catalyzes the formation of polysilicate. As described by Curnow et al, the silicatein gene was fused to the transmembrane domain of outer membrane protein A (OmpA), in order to display it at the surface of the cell. The fusion of silicatein and OmpA is constructed according to Francisco et al, consisting of the transmembrane domain of OmpA together with the signaling peptide and the first nine N-terminal amino acids of lipoprotein (Lpp). The coding sequence in this BioBrick is combined with the strong RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/composite_part/winner
Parameters
None