Regulatory
Part:BBa_K1895002
Designed by: iGEM16_Newcastle Group: iGEM16_Newcastle (2016-09-06)
Revision as of 09:50, 6 September 2016 by Jakeb (Talk | contribs) (Add summary of the biology behind the DnaK protein and promoter.)
dnaK Promoter
This is the dnaK promoter from E. coli K-12.
Usage and Biology
DnaK is a heat shock protein. It's role is that of a protein chaperone, it binds to proteins that have misfolded due to the stress. These proteins are then refolded in an HSP-mediated process or degraded (Arsène F, et al., 2000). Like other members of the Hsp70 family, dnaK in E. coli is upregulated by heat stress (Ritossa, F., 1996). Consequently its promoter must be stronger during heat shock to allow for overexpression. Although DnaK itself acts to shut off the heat shock response (Tilly, K. et al., 1983) we feel its promoter is a viable alternative heat shock promoter to the current registry entry, htpG (BBa_J45504).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//rnap/prokaryote/ecoli/sigma32
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/constitutive
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/constitutive
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