Coding

Part:BBa_K2012002:Experience

Designed by: Pan Chu   Group: iGEM16_HZAU-China   (2016-09-04)
Revision as of 06:51, 5 September 2016 by MinTT (Talk | contribs) (→‎Applications of BBa_K2012002)


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Applications of BBa_K2012002

Congo red staining For demonstrating expression of pleD, we used Congo red staining assay. As previous mentioned, high concentration of c-di-GMP could induce E. coli synthesize exopolysaccharides, and Congo red binding is a complex phenotype that reflects various outer membrane and surface properties including the presence of adhesive structures such as curli fimbria which are involved in biofilm formation. Wild type: DE3 contain pET28b plasmid, colony which was stained red color contain pET-pleD plasmid.

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