Coding

Part:BBa_K2042002

Designed by: Maria Bargués i Ribera   Group: iGEM16_Evry   (2016-09-02)
Revision as of 16:56, 4 September 2016 by RATOVOMANANA (Talk | contribs)


Pseudomonas sp. MBEL 6-19 PHA synthase 1

This is the coding sequence for a Polyhydroxyalkanoate synthase from Pseudomonas sp. MBEL 6-19. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer. This sequence includes four mutations which were found to increase yields of PLA (Yang et al.)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
    Illegal NgoMIV site found at 1339
    Illegal AgeI site found at 135
    Illegal AgeI site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//chassis/prokaryote/pputida
Parameters
None