Coding
Pct

Part:BBa_K2042000:Design

Designed by: Toky RATOVOMANANA   Group: iGEM16_Evry   (2016-08-19)
Revision as of 16:36, 4 September 2016 by RATOVOMANANA (Talk | contribs) (→‎References)


Propionate CoA transferase for P. putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1081
    Illegal BsaI site found at 1552


Design Notes

This sequence was codon optimized for Pseudomonas putida and silent mutation was introduce for removing restriction site. The iGEM Team Evry 2016 used this enzyme as the first enzyme in the pathway to produce Polylactic acid (PLA) in Pseudomonas putida.

Source

This sequence was synthetized by IDT

References

Biosynthesis of Polylactic Acid and Its Copolymers using Evolved Propionate CoA Transferase and PHA Synthase
Taek Ho Yang,1 Tae Wan Kim,1 Hye Ok Kang,1 Sang-Hyun Lee,1 Eun Jeong Lee,1 Sung-Chul Lim,1 Sun Ok Oh,1 Ae-Jin Song,2 Si Jae Park,1 Sang Yup Lee2,3
http://www.ncbi.nlm.nih.gov/pubmed/19937726

Microbial production of lactate-containing polyesters.
Yang JE1, Choi SY, Shin JH, Park SJ, Lee SY.
http://www.ncbi.nlm.nih.gov/pubmed/23718266