Coding

Part:BBa_K2042001:Design

Designed by: Toky RATOVOMANANA   Group: iGEM16_Evry   (2016-09-02)
Revision as of 12:50, 2 September 2016 by Registry (Talk | contribs)

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Pseudomonas resinovorans PHA synthase 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 87
    Illegal XhoI site found at 172
    Illegal XhoI site found at 1030
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 796


Design Notes

We used this sequence as second enzyme in order to produce PLA. In fact it will take lactyl coA as substract and polymerise it into PLA homopolymere



Source

This sequence was synthetized by IDT


References