Coding

Part:BBa_K118003

Designed by: Andrew Hall   Group: iGEM08_Edinburgh   (2008-07-24)
Revision as of 23:19, 29 August 2016 by RATOVOMANANA (Talk | contribs)

crtI coding sequence encoding phytoene dehydrogenase

This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene (Misawa, N., Nakagawa, N., Kobayashi, K., Yamano, S., Nakamura, K., and Harashima, K. 1990. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. Journal of Bacteriology 172, 6704-612). Two PstI sites were removed by site-directed mutagenesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 71
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Structure

File:BBa K118003-Evry2016.jpeg Crystal structure of Phytoene desaturase CRTI from Pantoea ananatis

[edit]
Categories
//function/biosynthesis/isoprenoid
//function/odor
Parameters
None