Generator

Part:BBa_K1979003:Design

Designed by: MA Xinyi   Group: iGEM16_SDSZ_China   (2016-08-26)
Revision as of 06:34, 26 August 2016 by Registry (Talk | contribs)

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PBP5 coding device (promoter+PBP5+T7 terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 135
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1178
    Illegal BamHI site found at 168
    Illegal XhoI site found at 1386
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 492
    Illegal AgeI site found at 1094
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Histidine tag is added for affinity purification to harvest the proteins.


Source

The PBP5 and terminator sequences are retrieved form GenBank, and the promoter sequence comes from the part BBa_J23101.


References