Plasmid_Backbone

Part:BBa_K2077000:Experience

Designed by: Danielle Bauhan, Alexander Lacrampe, Joseph Lee, and Richard Anthony   Group: iGEM16_RHIT   (2016-08-09)
Revision as of 00:56, 11 August 2016 by Xandafish (Talk | contribs) (→‎Applications of BBa_K2077000)


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Applications of BBa_K2077000

Figure 1. BY4741 yeast transformants with pSB416 GPD with mRPS12 TU as insert plated on CSM-URA and grown for five days.
Figure 2. BY4741 yeast without pSB416-GPD plated on CSM-URA and CSM-His media. Picture was taken after five days of incubation.


Figure 3. Top Left: BY4147 under fluorescent light. Top Right BY4147 under Normal light. Bottom Left: BY4741 with insert under fluorescent light. Bottom Right: BY4741 with insert under normal light


We have verified the function of the selectable markers, the promoter, the terminator, and the biobrick prefix and suffix in pSB416-GPD. BY4741 yeast that have their URA3 and HIS genes knocked out transformed with pSB416-GPD regained the ability to grow on CSM—URA as shown in Figures 1 & 2. Figure 3 demonstrates the function of the promoter, terminator and the biobrick prefix and suffix in pSB416-GPD. The yeast on the bottom row of the figure were transformed with pSB416-GPD with mls-yeGEP inserted into the vector using the bio brick prefix and suffix. The fluorescence demonstrated by the yeast verifies the function of the promoter and terminator in the vector.

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