Coding

Part:BBa_K1895998:Design

Designed by: Jake Burton   Group: iGEM16_Newcastle   (2016-07-21)
Revision as of 11:13, 21 July 2016 by Jakeb (Talk | contribs) (→‎Source)


SmtA metallothionein and LVA tag.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 93
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 93
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 93
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 93
    Illegal AgeI site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

[TODO]


Source

Variant of [Part:BBa_K519010 BBa_K519010] with protein degradation tag from Anderson et al. (1998)

References

Andersen, J.B., Sternberg, C., Poulsen, L.K., Bjørn, S.P., Givskov, M. and Molin, S., 1998. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), pp.2240-2246.