Coding
Part:BBa_K1895998
Designed by: Jake Burton Group: iGEM16_Newcastle (2016-07-21)
SmtA metallothionein and LVA tag.
This is part BBa_K519010, SmtA which is a metallothionein that can bind to heavy metal ions. This variant has an LVA degradation tag before the stop codon which reduces its half-life in the cell.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 93
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 93
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 93
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 93
Illegal AgeI site found at 121 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |