Coding

Part:BBa_K2019000:Design

Designed by: John Lazar   Group: iGEM16_Northwestern   (2016-07-15)
Revision as of 16:39, 15 July 2016 by Registry (Talk | contribs)

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Histidine Tagged WT Staphylococcus Aureus Cas9 Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 627
    Illegal BglII site found at 780
    Illegal BglII site found at 926
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 200
    Illegal AgeI site found at 2756
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We linearized a backbone with the tet promoter and then used gibson assembly to attach two gblocks with the Cas9 sequence.


Source

Tet promoter was used from the . SaCas9 was extracted from the genomic sequence of staphylococcus aureus and then codon optimized for E. Coli.

References